In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:
The M2-10B4 cell line is a clone derived from bone marrow stromal cells from a （C57BL/6J X C3H/HeJ）F1 mouse. The cells express laminin and collagen IV, but do not express collagen I or Factor VIII. The cells will support human and murine myelopoiesis in long term culture, and will support certain murine stromal cell dependent pre-B cell lines in vitro.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Subculturing:
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2001 recommended serum:ATCC 30-2020
References:
22620: Sutherland HJ, et al. Differential regulation of primitive human hematopoietic cells in long- term cultures maintained on genetically engineered murine stromal cells. Blood 78: 666-672, 1991. PubMed: 1713512 22769: Lemoine FM, et al. Partial characterization of a novel stromal cell-derived pre-B-cell growth factor active on normal and immortalized pre-B cells. Exp. Hematol. 16: 718-726, 1988. PubMed: 3261251 23070: Lemoine FM, et al. Autocrine production of pre-B-cell stimulating activity by a variety of transformed murine pre-B-cell lines. Cancer Res. 48: 6438-6443, 1988. PubMed: 3263188 23353: Lemoine FM, et al. Transformation-associated alterations in interactions between pre-B cells and fibronectin. Blood 76: 2311-2320, 1990. PubMed: 2147863
會(huì)員.png)
15
