CCL-56 Pt-K2 袋鼠腎細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
CCL-56 Pt-K2 袋鼠腎細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
CCL-56 Pt-K2 袋鼠腎細胞 的詳細介紹
| ATCC® Number: | CCL-56™ | Price: | $407.00 |
| Designations: | PtK2 （NBL-5） | | Depositors: | KH Whalen | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Potorous tridactylis （potoroo） | | Morphology: | epithelial
| | Source: | Organ: kidney
Disease: normal | | Cellular Products: | keratin | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Applications: | transfection host （Roche FuGENE® Transfection Reagents） | | Virus Resistance: | adenovirus 5; coxsackievirus B5; poliovirus 2 | | Age: | adult | | Gender: | male | | Comments: | The cells are positive for keratin by immunoperoxidase staining. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. | | Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 daysEvery 2 to 3 days | | Preservation: | Freeze medium: growth medium, 95%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003
recommended serum:ATCC 30-2020 | | References: | 26208: . . Genetics 51: 915, 1965. |
|
15
