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紅色熒光蛋白單克隆抗體說明書

時間:2025/8/1閱讀:43
分享:
產(chǎn)品編號bsm-33011M
英文名稱RFP Mouse mAb
中文名稱紅色熒光蛋白單克隆抗體
別    名Red fluorescent protein; Red fluorescent protein eqFP611; RFPTag; RFP-Tag; RFP Tag; RFP_ENTQU.  
產(chǎn)品類型標(biāo)簽抗體 
抗體來源Mouse
克隆類型Monoclonal
克 隆 號7A5
交叉反應(yīng)Recombinant protein
產(chǎn)品應(yīng)用WB=1:500-5000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量kDa
檢測分子量
細(xì)胞定位細(xì)胞核 細(xì)胞漿 
性    狀Liquid
濃    度1mg/ml
免 疫 原Recombinant Red fluorescent protein 
亞    型IgG
純化方法affinity purified by Protein G
緩 沖 液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產(chǎn)品介紹Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.Red Fluorescent Protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.

Function:
Pigment protein.

Post-translational modifications:
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen.


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