Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~780 nm.

AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

————————————————————————————

別藻藍蛋白 (APC) 是一種從藍綠藻螺旋藻中分離出來的藻膽蛋白。與其他藻膽蛋白一樣，APC 是熒光的，具有J高的吸收率和高量子效率。它是一種可以通過常規(guī)蛋白質(zhì)交聯(lián)技術(shù)輕松與抗體和其他蛋白質(zhì)連接而不改變其光譜特性的蛋白質(zhì)。APC-Cy7 是流式細胞術(shù)中常用的顏色。其主要吸收峰在 651 nm，發(fā)射峰在 ~780 nm。

艾美捷預激活APC-Cy7串聯(lián) 以促進 APC-Cy7 與抗體和其他蛋白質(zhì)（如鏈霉親和素和其他二級試劑）的串聯(lián)偶聯(lián)。該預激活APC-Cy7串聯(lián)已準備好進行偶聯(lián)，與傳統(tǒng)繁瑣的基于 SMCC 的偶聯(lián)化學相比，其產(chǎn)量要高得多。

艾美捷預激活APC-Cy7串聯(lián)普通儲備溶液制備：

將特定質(zhì)量的ReadiUse™ 預活化 APC-Cy7 Tandem復溶至給定濃度所需的適當溶劑體積。請注意，體積僅用于制備儲備溶液。

艾美捷預激活APC-Cy7串聯(lián)組件：

A：ReadiUse™ 預激活 APC-Cy7 串聯(lián) 1 瓶（1 毫克）

B：Buccutite™ MTA 1 瓶 (100 µg)

C：旋轉(zhuǎn)脫鹽柱 不包含

光譜特性：

消光系數(shù)（cm -1 M -1） 700000

激發(fā)（納米） 651

發(fā)射（納米） 779

儲存、安全和處理：

H-短語 H303、H313、H333

危險符號 XN

有可能的使用 僅供研究使用 (RUO)

R-短語 R20、R21、R22

UNSPSC 12171501