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Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.

產(chǎn)品概述

Product Description

Streptavidin is a protein that has similar binding properties to avidin. It is isolated from Streptomyces Avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin. Biotin is a 244 dalton water-soluble vitamin. Streptavidin has an extremely high binding affinity (Kd=10-15) for biotin.

Streptavidin-HRP (M00091) is coupled streptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated primary or secondary antibodies in enzyme immunoassay, immunoblotting, immunohistochemistry, flow cytometry, hybridoma screening systems. This conjugate also binds to nucleic acids labeled with biotinylated nucleotide analogs in Southern or Northern blot.

產(chǎn)品屬性

Formulation ：Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% Thimerosal

Reconstitution： Reconstitute the lyophilized power with 1.0 ml of deionized water (or equivalent) to a final concentration of 1 mg/ml.

Storage： The product remains stable in lyophilized form for at least 1 year if stored at 2-8°C, -20°C, or lower from the date of receipt. For longer storage, -20°C or below is recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles. If slight precipitation occurs upon prolonged storage, clarify the solution by centrifugation before use.

應用指導

Working concentrations for specific applications should be determined by titration assay. Appropriate concentrations will be affected by several factors including primary antibody, antigen amount, sensitivity of detection method, temperature, and incubation time, etc. The following dilution ranges are just a guideline:

Application： Recommended Usage

Dot blot ：1:2,000~1:10,000

Western blot： 1:2,000~1:20,000

ELISA ：1:5,000~1:20,000

Others： User optimized